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MedChemExpress
protein kinase c pkc inhibitors  Protein Kinase C Pkc Inhibitors, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/9%2E2+time-series+forecasting+procedure/Chelerythrine+chloride/pmc09560869-43-5-11 Average 94 stars, based on 1 article reviews
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Image Search Results
Journal: Translational Cancer Research
Article Title: Effect of IL-8 on hepatocellular carcinoma-associated metastasis by targeting MMP9 in mice
doi: 10.21037/tcr-22-37
Figure Lengend Snippet: Expression of MMP9 in IL-8 treated HCCLM6 cells. (A) Relative mRNA expression of MMP9. (B) Relative protein expression levels of MMP9 in IL-8 treated HCCLM6 cells. (C) Western blots show phosphorylated PKC, phosphorylated ERK1/2, and total proteins of PKC or ERK1/2 at the indicated times. α-tubulin was used as a loading control. (D) The PKC inhibitor chelerythrine chloride (20 µM) and ERK1/2 inhibitor PD98059 (40 µM) significantly decreased the mRNA levels of MMP9 in IL-8-treated HCCLM6 cells. (E) Western blot assay was used to assess the protein expression levels of MMP9 in IL-8-treated HCCLM6 cells. The data are presented as mean ± SD. *, P<0.05; **, P<0.01, compared with the blank group. MMP9, matrix metallopeptidase 9; IL-8, interleukin-8; HCCLM6, hepatocellular carcinoma LM6; PKC, protein kinase C; ERK1/2, extracellular regulated protein kinases 1/2; SD, standard deviation.
Article Snippet: To evaluate the effects of
Techniques: Expressing, Western Blot, Control, Standard Deviation
Journal: Translational Cancer Research
Article Title: Effect of IL-8 on hepatocellular carcinoma-associated metastasis by targeting MMP9 in mice
doi: 10.21037/tcr-22-37
Figure Lengend Snippet: Effects of PKC and ERK1/2 inhibitors on pro-colonization induced by IL-8 in the orthotopically implanted mice. (A) Representative images of live tissues in the orthotopically implanted mice that were treated with PKC and ERK1/2 inhibitors. (B) Hematoxylin/eosin-stained images and the number of metastases of liver or lung tissues isolated from the PKC and ERK1/2 inhibitors treated mice as indicated. Scale bars =400 µm. (C) MMP9 expression of live tissues was measured by qRT-PCR in PKC and ERK1/2 inhibitors-treated orthotopically implanted mice. (D) MMP9 expression of live tissues was measured by immunohistochemical staining in PKC and ERK1/2 inhibitors-treated orthotopically implanted mice. Scale bars =100 µm. Data are presented as mean ± SD. *, P<0.05; **, P<0.01, compared with the IL-8 group. PKC, protein kinase C; ERK1/2, extracellular regulated protein kinases 1/2; IL-8, interleukin-8; IOD, integral optical density; MMP9, matrix metallopeptidase 9; qRT-PCR, quantitative real-time polymerase chain reaction; SD, standard deviation.
Article Snippet: To evaluate the effects of
Techniques: Staining, Isolation, Expressing, Quantitative RT-PCR, Immunohistochemical staining, Real-time Polymerase Chain Reaction, Standard Deviation
Journal: The Journal of Clinical Endocrinology and Metabolism
Article Title: Activin-A and Myostatin Response and Steroid Regulation in Human Myometrium: Disruption of Their Signalling in Uterine Fibroid
doi: 10.1210/jc.2010-0501
Figure Lengend Snippet: A and B, Activin and myostatin levels of mRNA expression in eight paired fibroid and myometrial specimens. C–F, Levels of mRNA expression in eight paired fibroid and myometrial specimens for the four receptor genes ALK4, ALK5, ActRII, and ActRIIB. Graphs on column I are line graphs linking paired specimens, and those on column II are box-and-whisker plots. In column III is reported the correlation of activin-A with its receptors, whereas in column IV is reported the correlation of myostatin with its receptors. The nonparametric correlation (Spearman) revealed no correlation of both activin and myostatin levels with receptor levels. *, P < 0.05.
Article Snippet: All results were normalized against either β-actin and hypoxanthine-guanine phosphoribosyl transferase expression to correct for differences in concentration of the starting template. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene name Alias Gene symbol Reference sequence Assay ID Amplicon length Inhibin, βA EDF INHBA {"type":"entrez-nucleotide","attrs":{"text":"NM_002192.2","term_id":"62953137","term_text":"NM_002192.2"}} NM_002192.2 Hs00170103_m1 61 FRP Follistatin-like 3 (secreted glycoprotein) FLRG FSTL3 {"type":"entrez-nucleotide","attrs":{"text":"NM_005860.2","term_id":"95104789","term_text":"NM_005860.2"}} NM_005860.2 Hs00610505_m1 84 FSRP Actin, β PS1TP5BP1 ACTB {"type":"entrez-nucleotide","attrs":{"text":"NM_001101.3","term_id":"168480144","term_text":"NM_001101.3"}} NM_001101.3 Hs99999903_m1 171 Myostatin GDF8 MSTN {"type":"entrez-nucleotide","attrs":{"text":"NM_005259.2","term_id":"149408158","term_text":"NM_005259.2"}} NM_005259.2 Hs00193363_m1 118 Activin-A receptor, type IIA ACTRII ACVR2A {"type":"entrez-nucleotide","attrs":{"text":"NM_001616.3","term_id":"65508448","term_text":"NM_001616.3"}} NM_001616.3 Hs01012007_m1 110 ACVR2 Activin-A receptor, type IB ACTRIB AcvR1B {"type":"entrez-nucleotide","attrs":{"text":"NM_004302.3","term_id":"33598913","term_text":"NM_004302.3"}} NM_004302.3 Hs00923299_m1 74 ACVRLK4 ALK4 SKR2 TGF, β receptor 1 AAT5 TGFBR1 {"type":"entrez-nucleotide","attrs":{"text":"NM_004612.2","term_id":"66346739","term_text":"NM_004612.2"}} NM_004612.2
Techniques: Expressing, Whisker Assay
Journal: The Journal of Clinical Endocrinology and Metabolism
Article Title: Activin-A and Myostatin Response and Steroid Regulation in Human Myometrium: Disruption of Their Signalling in Uterine Fibroid
doi: 10.1210/jc.2010-0501
Figure Lengend Snippet: TaqMan gene expression assays (Applied Biosystems) used to perform the real-time PCR
Article Snippet: All results were normalized against either β-actin and hypoxanthine-guanine phosphoribosyl transferase expression to correct for differences in concentration of the starting template. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene name Alias Gene symbol Reference sequence Assay ID Amplicon length Inhibin, βA EDF INHBA {"type":"entrez-nucleotide","attrs":{"text":"NM_002192.2","term_id":"62953137","term_text":"NM_002192.2"}} NM_002192.2 Hs00170103_m1 61 FRP Follistatin-like 3 (secreted glycoprotein) FLRG FSTL3 {"type":"entrez-nucleotide","attrs":{"text":"NM_005860.2","term_id":"95104789","term_text":"NM_005860.2"}} NM_005860.2 Hs00610505_m1 84 FSRP Actin, β PS1TP5BP1 ACTB {"type":"entrez-nucleotide","attrs":{"text":"NM_001101.3","term_id":"168480144","term_text":"NM_001101.3"}} NM_001101.3 Hs99999903_m1 171 Myostatin GDF8 MSTN {"type":"entrez-nucleotide","attrs":{"text":"NM_005259.2","term_id":"149408158","term_text":"NM_005259.2"}} NM_005259.2 Hs00193363_m1 118 Activin-A receptor, type IIA ACTRII ACVR2A {"type":"entrez-nucleotide","attrs":{"text":"NM_001616.3","term_id":"65508448","term_text":"NM_001616.3"}} NM_001616.3 Hs01012007_m1 110 ACVR2 Activin-A receptor, type IB ACTRIB AcvR1B {"type":"entrez-nucleotide","attrs":{"text":"NM_004302.3","term_id":"33598913","term_text":"NM_004302.3"}} NM_004302.3 Hs00923299_m1 74 ACVRLK4 ALK4 SKR2 TGF, β receptor 1 AAT5 TGFBR1 {"type":"entrez-nucleotide","attrs":{"text":"NM_004612.2","term_id":"66346739","term_text":"NM_004612.2"}} NM_004612.2
Techniques: Expressing, Sequencing, Amplification